How to clean subfloor from pet urine

Using Enzymatic Cleaners One solution for removing pet urine odor from wood sub-floor is to apply an enzymatic pet cleaner to the soiled areas, and allow them to dry thoroughly for 24 hours. It's important that you allow the area to dry completely in order to give the enzymes time to remove the stain and odor.

Pour the hydrogen peroxide over the stain and then cover it with a clean cloth soaked in hydrogen peroxide. Cover the cloth with plastic wrap, weighting it with a book if necessary. Allow this to sit overnight or for at least 8 hours. The hydrogen peroxide should remove any stains and eliminate the urine odor.

WHITE VINEGAR AND GRAPEFRUIT OIL Mix one cup of vinegar into a warm water-filled bucket and the add some drops of grapefruit oil to the solution to get rid of the odor. Scrub the floor with this safe cleaning solution, concentrating on the most prominent spots. As you scrub, the odor and stains should be disappearing.

Mix a one-to-one solution of white vinegar and water. Using a sponge, rub the solution onto the stain. Let it sit for 5-to-10 minutes, and then wipe it up with a clean, dry towel. Some experts advise using a more diluted formulation of 1/2-cup vinegar to one gallon of warm water.